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The Microscope

Lesson 6 from: Beyond Macro Photography: Into the Microscopic World

Charles Krebs

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Lesson Info

6. The Microscope

Lesson Info

The Microscope

And a microscope. But big difference is, this is all in one. I mean, you got the focusing stage, you know, built in. You've got a nice x y movement. You can see what you're looking at. You got the object is on a church. So it's, essentially, ah, these tabletops setups, but really refined, really fine and really, really fine movements. Um and and allows you to work at even higher magnification. You could do things like the grooves on a record. Okay, old thirty three rpm record, that's, what the grooves look like, ok, you can get it instead of doing the insect, you can just you can do. Ah, details on the leg. This is the leg of, ah, aquatic insects. Anyhow, you'll find creatures that you never even knew existed in amoeba that makes a shell. These are things you can't see. Now. This is this is the next level that we've gone into the microscope, little little little creatures that are feeding constantly with the cilia that move around, dia tom's that are that are just gorgeous, you know, u...

m aliens from outer space or right there in your backyard in your birdbath okay this basically is the microscope these air the components it's easy to see this you have the objectives you have the viewing eyepieces cameras mounted up top you have a stage focusing everything is there all put together for your very precise very fine movements the lighting typically is trance illuminated in this type it comes through the base and you have this one has a vertical illuminated on it so what can you do with the microscope? The lighting is maybe a little more limited because with the others pictures we're lighting it like a little mini studio we've got our thinks it up and we got our lights and are expensive ikey lights you know all set up so you're lighting it like a little studio subject here you use lighting situations or lighting setups that are provided by the manufacturer this is the common one this is bright field every microscope gets going tohave 00:02:06.334 --> 00:02:08. bright field so this is a mosquito wing on bright 00:02:08.94 --> 00:02:12. field you have a white background okay and the light 00:02:12.31 --> 00:02:15. comes from the bottom goes to the subject dark field 00:02:15.83 --> 00:02:19. there's another very common easy to set up doesn't 00:02:19.33 --> 00:02:21. really you could get a condemned a special condenser 00:02:21.61 --> 00:02:22. for you could do it yourself 00:02:24.22 --> 00:02:26. like I said a bright field you have a black background 00:02:26.55 --> 00:02:29. on this subject still illuminated very dramatic. It 00:02:29.37 --> 00:02:32. really brings out certain subjects. This is a snail 00:02:32.62 --> 00:02:35. eggs on dh this is a dark dark field so this is a 00:02:35.81 --> 00:02:38. a lighting technique you can modify dark field a little 00:02:38.9 --> 00:02:43. bit and it's called grinberg where instead of having 00:02:43.22 --> 00:02:45. the black background you have you can have a colored 00:02:45.99 --> 00:02:47. background this is 00:02:49.72 --> 00:02:52. mosquito larva with a dark blue background sort of 00:02:52.33 --> 00:02:55. dark field but it's really ryan berg crossed polarizer 00:02:56.52 --> 00:02:58. a lot of materials you look at a mexican will buy 00:02:58.36 --> 00:03:00. refrigerate if you put a polarizing on top of polarized 00:03:00.98 --> 00:03:02. on the bottom and jiggle it around a little bit things 00:03:02.93 --> 00:03:06. light up like the muscles in an aquatic insects okay 00:03:07.42 --> 00:03:10. vitamin c crystals are very buyer a fringe and see 00:03:10.85 --> 00:03:13. you get these dramatic colors blue food dye 00:03:15.32 --> 00:03:19. crystallised by referencing crystals cross polarizer 00:03:20.52 --> 00:03:25. plant aquatic plants cell walls by refrigerant this 00:03:25.39 --> 00:03:27. is d I see this is now a little more exotic this a 00:03:27.99 --> 00:03:31. little more expensive now a little more that's a lot 00:03:31.38 --> 00:03:33. more expensive but it's a very nice type of illumination 00:03:33.6 --> 00:03:36. it's something you know serious you know biological 00:03:36.81 --> 00:03:38. microscopes love and strive for 00:03:40.22 --> 00:03:43. but it's a little bit hard to come buy used and it's 00:03:43.55 --> 00:03:46. quite expensive new so we don't dwell on that too 00:03:46.13 --> 00:03:49. terribly much this is phase contrast used to be used 00:03:49.34 --> 00:03:52. a lot it's not the greatest for photography but it 00:03:52.1 --> 00:03:55. does allow you to see things that are usually very 00:03:55.43 --> 00:03:56. very thin 00:03:57.52 --> 00:04:01. like amoebas and things like that bacteria but photographically 00:04:01.45 --> 00:04:03. I I just don't use it that much anymore. I'm not too 00:04:03.49 --> 00:04:07. excited. And then we have the reflected where we can 00:04:07.41 --> 00:04:09. take the kind of thing we're doing with these tabletops 00:04:09.56 --> 00:04:11. setups to sort of the next level. A. Uh the camera chip. You can see the rgb filters on the lower left and somebody electronics in the upper right.

Class Materials

Bonus Materials with Purchase

Beyond Macro Photography: Into the Microscopic World Slides

Ratings and Reviews

a Creativelive Student

Like the other reviewers I enjoyed this class but would like a very in-depth part 2. He just mention lighting, a ping pong ball and a white dome. I'd like to learn how those are used. Where do you get the dome, what material is it, etc. I'd also like to learn about his specimens. While he mentions what they are, how do you use the water from your bird bath? Does it go on a slide? Please do a part 2. Thanks!

Sara Zancotti

Super interesting but too short. I would love to have a longer course with Charles! Thank you Charles, you made me remember my childhood. I had a simple little microscope that my father gave me back in the 90s - sweet memories!


I found this course very interesting. I, like other reviewers, would have enjoyed a longer presentation with more hands on instruction. I'd like something that goes from A-Z, everything from acquiring your subjects (Are they purchased, caught, frozen, pinned, etc) to the photographing of the subject (lighting, etc.), to the end result. The focus stacking was the most hands on portion while other sections were just informative. I really enjoyed the class and Mr. Krebs knows his stuff but I would pay to have another more in depth and hands-on kind of class with Mr. Krebs,

Student Work