Microscope Objectives: The Set Up
Okay, how can I use these microscope it's a no brainer. You screwed it to hurt and you use it on a camera. Well, there's one very simple way out of thanks to ebay and in chinese manufacturers we've got some of these guys to make adapters for he's different microscope projectors. And so here is the stream microscope objectives there tucked into uh um these adapters and they could be attached directly to the front of the camera lens. Now this is an infiniti. What does it mean? What is infinite atlanta needs it needs another optic between the lens of lo and behold, you can actually use a camera and he's like this is this requires a two hundred millimeter lens. This is a fifty five to torch a millimeter lens attached with this adapted the adapters or I don't know what ten fifteen bucks I got the lands it's a kit lens I have to assume the lens after two hundred because that's what the objective was designed for, but I'm often working in war from working at five extra ten x or y or whatever ...
it is just a simple flat glass list adapter here I'm using a camera lens as the two blends um here I've gotten a little more sophisticated I'm using a bellows gives me a little more versatility on an indoor set up and I have actually put a nikon to blends here so I can attach my affinity optics to the bellows and use them and I have the land between here we have ah a finite lands that just needs extension tube's know optics in between here these air like set eight eight bucks chinese extension tube's that's all I need in the lands with the adaptor on front and I'm ready to roll I'm ready to work a te next to do any of these types of pictures that I kind of showed you here tonight and again I get a little have the bellows and I get a little get a little more sophisticated and you can put the finite lands on a bellows pure extension that's the only reason it's there what thing I will say if you do try this and you get these adapters double check if you have a lens where some lenses that the front element really comes very close to the filter er in front of the lens the front element is not recessed it all some of these adapters when you screw the objective and they get out of it so I don't want you to screw this in and have they objective scratching the front of the lens you get space rings if needed just make sure that the objective is not going to hit the front of your lens depends on the adaptor that you get the other thing is always, always look at the back. A lot of the objectives have very shiny rims that show through, even cover them with a little piece of black tape or cardboard or something. You want to try to reduce flare as much as possible. Now I don't wanna get into ah whole lot here but of specifics, but I hate to throw you out there. Um, without any sense at all of what's what's good and what's not good. So there's some recommendations, these the ones I've used these are ones that worked really well. Mitutoyo is an industrial lands manufacturer. They have a whole different siri's this particular syriza long work in distance siri's is superb. I mean, this is sort of this is sort of the cream. This is what? You know, if you can if you can afford it. This is what you want. The working distances are ex superior, their remarkable. The optics are remarkably good. The prices are remarkable. Um, they're available used their current manufactures, but it'll varia five x brand new might be eight hundred dollars uh twenty x brand new and I'm guessing him, you know, maybe sixteen, seventeen hundred dollars on a used market. You'll see him very often for somewhere between three and six hundred dollars, okay? The olympics plant floor rights o r ones I used to and they're also pretty pricey. They were also very, very good. You'll notice that the working distances a lot less, but they're they're very they're current there, martin lenses. They're very good. These air, both infinity types in older siri's are are a nikon implant. Sacramento these aren't the acrobats, so they're not the super highly corrected, but they're very good and they're there. There are a lot of them out there and they used market uh on their available, you know, five tens twenties notice on the twenty and forty I said, long working this it's an extra long working distance l w do there's no hard and fast rule as teo uh what that means, but it typically denotes a longer working distance. You have to just track down the specs. Um, nikon cf serious there's a couple of old biological objectives that that work well there and, uh, for some reason, powerpoint just got really nasty on my my tabs and spacing on that slide and these air no worthy. I just want to point these two atoms making up. These are new nikon lenses. They're infinity lenses, they're very, very good, but they're they're sort of started ledges and there cheap for newlands. The four ex that I list their show there is about seventy five bucks knew the middle one. The ten x is about eighty five. Ninety bucks knew and the one below that is about two hundred fifty dollars. New that's. Kind of a step up. You could see the working distance is six point seven million that's a that's a little too close for my taste, but it'll work. And the other piece of equipment that you're going to need some sort of focusing platform to do, um to enable you to move the camera forward backward in very small increments. Okay, because we shoot a stack that's what we have to do, we have to gradually move the camera, take a picture, move it, move it, move it! Now you're not moving it that much you moving it with in my crones with a little micrometer scale over here. So the typical focusing stages that we have for a close up work of far too crude for this, you have to you have to find something, and this is where you need to look a little bit. I use this is ah, olympus be h m j focusing rack you confined separately, used a lot of people will use in the optical industry. They have these translation stages. This is newport they make a lot of this other manufacturers to I just pointed out the newport won and the camera gets mounted on that and you adjust it with a micrometer this is ah cognisant makes is from called a stack shot this is actually designed for taking stacks it's got a little computerized control you basically tell it where to start where to stop hit a button and it moves the camera takes a picture lose the camera takes a picture you can go have a sandwich while your photographic stack instead of sitting there turning this and so this is you know this is just basically the setups that I'll use and with these microscope lenses again these are these are the types of pictures I spent a lot of time with another anti but these are the type of pictures you can expect when you get into the ten x twenty ex um fifteen this actually fifty x these I believe are ten x okay um this is a fifty x and we're not going to about lighting at all but just to show you how this was illuminated the wing scales are inside that little little cup which was cut out of a ping pong ball and coming in from the right is on I ke a lamp cost nine ninety nine at ike okay little l e d lamp I use I have three four those I use those a lot is the peacock feather where I needed a lot? Look, a lot more diffuse light, so I covered it with a dome. Okay, okay, on, uh, illuminated from the outside with those aki alights.